Maitake (Grifola frondosa) Liquid Culture Syringe

The Maitake (Grifola frondosa) Liquid Culture Syringe contains liquid mycelium prepared from a pure mushroom culture. The cultures are made using high-quality malt and yeast extracts. Liquid mycelium based on pure mushroom cultures is much more effective than spore-based cultures. The main advantage of liquid cultures is that they spread more evenly through a jar of grain, ensuring faster colonization. The contents of the syringe are intended solely for colonizing substrates and not for direct consumption.

The Maitake mushroom has played a role in cultural and traditional practices for centuries, especially within Asian communities. Its nickname and the appreciation shown upon its discovery reflect its special status. Its popularity remains high, both in the kitchen and as a supplement.

Content

These items are extra-packed in a resealable bag to prevent contamination:

• 1x 20 ml liquid culture in a syringe
• 1x alcohol swab
• 1x sterile needle

Use

• Add 1–2 ml per 1 L jar of sterilized grain
• Shake well before use
• Be patient: Maitake mycelium grows slowly and sometimes irregularly. It may seem like nothing is happening, but growth has likely already begun.

After use

• Store in the refrigerator again
• Dry the needle thoroughly with a new alcohol swab before replacing the protective cap to prevent rust
• Optional: briefly heat the needle with a lighter to evaporate residual moisture. Allow to cool before replacing the cap
• Place the liquid culture in a ziplock bag and return it to the refrigerator

These steps ensure the culture remains effective and usable for as long as possible.

What substrate does Maitake grow on?

For large-scale cultivation, a specific bulk substrate is required. Various types of hardwood (such as poplar, oak, alder, maple, birch, ash, beech, elm) mixed with bran are suitable. A popular alternative is the “master’s mix”: an equal ratio of hardwood sawdust and soy hulls. You can also grow naturally outdoors on hardwood logs.

Phase 1: Mycelium growth

• Temperature: 21–24°C
• Humidity: 95–100%
• Duration: 14–30 days, followed by 30 days of rest
• Light: n/a

Phase 2: Primordia formation – part 1

• Temperature: 10–15.6°C
• Humidity: 95%
• Duration: 5–10 days
• Light: 100–500 lux

Phase 3: Primordia stage – part 2

• Temperature: 10–15.6°C
• Humidity: 90–95%
• Duration: 10–14 days
• Light: 100–500 lux

Phase 4: Fruiting and harvest

• Temperature: 13–16 (max. 18)°C
• Humidity: 75–85%
• Duration: 14 – 21 days
• Light: 500–1,000 lux
• Harvest cycles: 2 flushes, every 3–4 weeks

Storage advice

• Use the liquid culture within 2 months
• Store in the refrigerator or another cool, dark place
• Avoid exposure to UV light